To reliably distinguish different phases of the hair cycle e.g. late catagen and telogen by stero-microscopy alone is challenging and requires histological analysis. In order to tackle this problem, we have established an intravital technique, based on meythelene blue staining, that can be applied to distinguishing late catagen from telogen hair follicles.
Our established method is simple, fast and economical. Correct and efficient hair stage classification methodology can advance human hair research that is clinically relevant. Based on this technique, we are now able to offer a telogen hair follicle ex vivo organ culture model to investigate whether compounds/formulations activate bulge stem cells and promote changes in the signaling involved in telogen-to-anagen transition.