In this study a skin organ culture model using lesional skin of Hidradentitis Suppurativa patients was applied. We could show that the inhibition of IL-17A by Secukinumab modulates pathways associated with IL-1 and TNFα signaling. Consistent with previous findings, IL-17RA/IL-17RC receptor activation induces IL-1 and TNFα signaling, and the expression of pro-inflammatory mediators is modulated by IL-17A interaction with both pathways.