Stem cell function

We use ex vivo organ culture of human hair follicles and human full thickness skin to investigate whether compounds/formulations (also via topical application) promote or inhibit stemness, differentiation, apoptosis, senescence/aging and/or epithelial-mesenchymal transition in stem cell compartments. Specifically, as standardized readout parameters, we evaluate the following by immunohistology/quantitative (immuno-)histomorphometry (for details on our techniques, please click here):

In addition, using RNAseq, qRT-PCR, and/or in situ hybridization, we can analyse the expression of molecules involved in stem cell physiology, and assess these within specific compartments from skin or hair tissue sections following laser capture microdissection.

Additional readout parameters are available, and customized experiments can be designed to meet the needs of our customers.

Bertolini et al., 2020, Gherardini et al., 2019,Cheret et al., 2020, Imanishi et al., 2018, Purba et al., 2018, Ali et al., 2017, Purba et al., 2014, Ernst et al., 2013