Techniques

Our team comprises experienced scientists and research technicians from diverse research backgrounds who are well trained in various methods and cutting-edge techniques to accelerate hair and skin research. Based on the custom-built projects, different methodologies and techniques can be utilized.

These include (but are not limited to):

Image

Morphology

Bright-field microscopy

High-resolution imaging

Optical coherence tomography (collaboration with Biomedical Technology Center)

Nano-texture of corneocytes

Atomic force microscopy (collaboration with Dermatact)

Culture media analysis

ELISA, LDH release, Cyto/Chemokine release, Gluthatione assay, etc.

Protein expression

Immuno-histochemistry, Western blots, FACS etc.

Enzymatic activity

In situ zymography, In vitro zymography

Gene expression

In situ hybridization, microarray, qRT- PCR, RNAseq

Metagenomics

16S sequencing, ITS sequencing (microbiome studies), In situ hybridization (e.g. 16S) etc.

Gene silencing

siRNA technique

Bioinformatics

PCA analysis, network modelling, pathways analysis, etc.

Spectroscopy

Lipidomics (collaboration with Lipotype), metabolomics, etc.

Isolation of cell compartments or single cells

Laser capture microdissection, MACS, FACS cell sorting

Exposure to environmental stressors

UVA and/or UVB irradiation, treatment with pollutants (Benzoapyrene, particulate matter)

Please do not hesitate to contact us to enquire and discuss more about different techniques that can be custom applied for your project.