Techniques
Our team comprises experienced scientists and research technicians from diverse research backgrounds who are well trained in various methods and cutting-edge techniques to accelerate hair and skin research. Based on the custom-built projects, different methodologies and techniques can be utilized.
These include (but are not limited to):
Morphology
Bright-field microscopy
High-resolution imaging
Optical coherence tomography (collaboration with Biomedical Technology Center)
Nano-texture of corneocytes
Atomic force microscopy (collaboration with Dermatact)
Culture medium analysis
ELISA, LDH release, Cyto/Chemokine release, Gluthatione assay, etc.
Protein expression
Immuno-histochemistry, Western blots, FACS etc.
Enzymatic activity
In situ zymography, In vitro zymography
Gene expression
In situ hybridization, microarray, qRT- PCR, RNAseq, in situ transcriptomics etc.
Microbiome analysis
Metagenomics, 16S RNA sequencing, ITS sequencing (microbiome studies), In situ hybridization (e.g. 16S) etc.
Gene knock-down
siRNA technique
Bioinformatics
PCA analysis, differential gene expression, network modelling, heatmap, pathways analysis, etc.
Multi-Omics analysis
Protemics, Lipidomics (collaboration with Lipotype), metabolomics, etc.
Analysis of selected compartments
Laser capture microdissection, MACS, FACS cell sorting
Single cell analysis
10x Genomics, MACS, Laser capture microdissection, FACS cell sorting.
Identification of chemical compounds
Histochemistry
Please do not hesitate to contact us to enquire and discuss more about different techniques that can be custom applied for your project.