Theophylline exerts complex anti-aging and anti-cytotoxicity effects in human skin ex vivo.
Bertolini. M et al., Int J Cosmet Sci, 2020
The detailed insights of the effect of theophylline on human skin are poorly understood. Here, we show that theophylline possesses several striking complex skin-protecting properties in human skin by employing the clinically relevant human full-thickness skin ex vivo organ culture model. Our study encourages further examination of theophylline as a topical candidate for anti-aging treatment.
Tissue-resident macrophages can be generated de novo in adult human skin from resident progenitor cells during substance P-mediated neurogenic inflammation ex vivo.
Gherardini. J et al., PloS One, 2020
Our current understanding of Monocyte (MO)-derived macrophages (MACs) origin and their differentiation in peripheral tissues is limited to murine studies and the existence of such activities in human skin remains elusive. By applying our established full-thickness skin organ culture, the results provide the first insights that de novo generation of human dermal CD68+ MACs from an expanding pool of resident CD34+ mesenchymal stem cells is possible also in human skin. Therefore, it would be intriguing to examine the MAC pool in skin diseases (dermatoses) and develop therapeutics targeting pathological intradermal MAC differentiation processes.
Non‐neuronal kappa‐opioid receptor activation enhances epidermal keratinocyte proliferation, and modulates mast cell functions in human skin ex vivo.
Cheret J et al., J Dermatol, 2020
It still remains elusive how Kappa‐opioid receptor (KOR) agonists impact on human skin and dermal mast cells (MCs) ex vivo, in the absence of any functional innervation of sensory nerve fibres. In this study, KORA (5a) was administrated “systemic application mode” to the clinically relevant human skin organ culture. The results show significant upregulation of the proliferation of epidermal keratinocytes and regulates mast cell functions. Our study suggests that KORA (5a) as a promising candidate to modulate MC‐dependent neurogenic skin inflammation for the management of inflammatory dermatoses.