We collaborate with Lipotype GmbH, Dresden Germany and have established a novel technique to perform high-resolution shotgun lipidomics analysis on micro-dissected human hair follicles.
Together with our partner, we can investigate effectively whether any compound or formulations affect the lipid composition of the hair follicle and developing hair shaft, by employing hair follicle organ culture and applying mass spectroscopy and double mass spectroscopy of hair membrane and storage lipids. In addition, we also offer complementary lipid analysis: quantification of fatty acids, glycerol, and triglycerides in the culture media. Also, histochemistry of total lipids (Oil red, Sudan black B), free fatty acids (Holczinger copper rubeanic acid staining), cholesterol and cholesteryl ester (Perchloric acid-naphthoquinone reaction). We perform immunohistology and quantitative histomorphometry of specific hair lipid synthases and transporters.
Furthermore, by applying our state-of-art laser capture microdissection method, we have the expertise to analyze integral hair lipids from different compartments (e.g. hair shaft, inner root sheath) from skin or hair tissue sections.
Lipidomics analysis is crucial to understand the physiology and pathology of hair, such as hair barrier, hair fiber structure, hair loss.
Please do not hesitate to contact us for a customized study to apply our state-of-the-art technique.
Our methodological approach for “Lipidomics analysis of human hair follicle and hair shaft” download here