We use ex vivo organ culture of human hair follicles and human full thickness skin, and humanized mouse in vivo models to investigate whether compounds/formulations (also via topical application) protect from experimentally-induced hair follicle immune privilege collapse (relevant in hair follicle immunological disorders). Specifically, as standardized readout parameters, we evaluate the following by immunohistology/quantitative (immuno-) histomorphometry (for details on our techniques, please click here).
- IFNγ signalling activation
Expression of proteins, and enzymes associated with the maintenance of hair follicle immune privilege
- Expression of growth factors associated with the maintenance of hair follicle immune privilege
- Number and phenotype of immune cells
In addition, using RNAseq, qRT-PCR, and/or in situ hybridization, we can analyse the expression of molecules involved in immune privilege, and assess these within specific compartments from skin or hair tissue sections following laser capture microdissection.
Additional readout parameters are available, and customized experiments can be designed to meet the needs of our customers.
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