We use primary epidermal keratinocytes, 2D/3D cell models, ex vivo human full thickness skin and humanized mouse in vivo models to investigate hydration and skin barrier defects and whether compounds/formulations (also via topical application) can reinforce the skin barrier. Specifically, as standardized readout parameters, we evaluate the following by immunohistology/quantitative (immuno-) histomorphometry (for our techniques, please click here).
- Epidermal thickness
- Defects on skin surface
- Diffusion of fluorescent dye through the skin
- Epidermal keratinocyte differentiation
- Expression of tight, and gap junctions
- Expression of aquaporins
- Expression of lipids
Now available: Nanotexture of corneocytes by atomic force microscopy.
In addition, using qRT-PCR and/or in situ hybridization, we can analyse the expression of genes involved in skin barrier integrity, and assess these within specific compartments from skin tissue sections following laser capture microdissection.
Our methodological approach for "analysing effects on barrier integrity ex vivo" download here
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