Cytotoxicity, proliferation & apoptosis
We use cell lines (HaCat), primary epidermal keratinocytes, dermal fibroblasts, hair follicle keratinocytes (outer root sheath) and skin immune cells, as well as ex vivo organ culture of human hair follicles and human full thickness skin to investigate whether compounds/formulations (also via topical application) inhibit cell proliferation, induce cytotoxicity, or protect from experimentally-induced cytotoxicity. Specifically, as standardized readout parameters, we evaluate the following by colorimetric assays, and immunohistology/quantitative (immuno-)histomorphometry (for details on our techniques, please click here):
Cell viability (MTT assay, lactate dehydrogenase assay)
Cell proliferation and apoptosis (using different markers detecting different stages of cell cycles and apoptosis)
Cell necroptosis (programmed inflammatory cell death)
Formation of melanin clumping (hair follicle only)
DNA damage (H2A.X, 8-OH-dG)
In addition, using RNAseq, qRT-PCR, and/or in situ hybridization, we can analyse the expression of molecules involved in anagen/catagen conversation, and assess these within specific compartments from skin or hair tissue sections following laser capture microdissection.
Additional readout parameters are available, and customized experiments can be designed to meet the needs of our customers.
Our methodological approach for “Investigating effects on skin and hair cytotoxicity” download here
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